Integrated manufacturing of REciclable multi-material COmposites for the TRANSport sector

Graphical Abstract and Lay SummaryHeme is a key ingredient required to mimic the color and flavor of meat in plant?based alternatives. Herein, the efficient synthesis of heme was achieved by reconstructing the heme biosynthetic pathway in S. cerevisiae. A glucose?limited fed?batch fermentation using the best strain, the HMX1?deficient strain overexpressing HEM3, HEM12, and PUG1, resulted in 28 mg/L of heme.ABSTRACTHeme is a key ingredient required to mimic the color and flavor of meat in plant?based alternatives. This study aimed to develop a yeast?based microbial cell factory for efficient and sustainable production of heme. To this end, first, Hem12p (uroporphyrinogen decarboxylase) was identified as the rate?limiting enzyme in the heme biosynthetic pathway present in Saccharomyces cerevisiae D452?2. Next, we investigated the effects of disruption of the genes involved in the competition for heme biosynthesis precursors, transcriptional repression, and heme degradation (HMX1) on heme production efficiency. Of the knock?out strains constructed in this study, only the HMX1?deficient strain produced heme at a higher concentration than the background strain without gene disruption. In addition, overexpression of PUG1 encoding a plasma membrane transporter involved in protoporphyrin IX (the precursor to heme biosynthesis) uptake led to a significant increase in intracellular heme concentration. As a result, among the various engineered strains constructed in this study, the ?HMX1/H3&12 + PUG1 strain, the HMX1?deficient strain overexpressing HEM3, HEM12, and PUG1, produced the highest concentration of heme (4.6 mg/L) in batch fermentation, which was 3.9?fold higher than that produced by the wild?type D452?2 strain. In a glucose?limited fed?batch fermentation, the ?HMX1/H3&12 + PUG1 strain produced 28 mg/L heme in 66 h.

» Publication Date: 09/10/2024

» More Information

« Go to Technological Watch