Integrated manufacturing of REciclable multi-material COmposites for the TRANSport sector
Fabrication of pH?sensitive double cross?linked sodium alginate/chitosan hydrogels for controlled release of amoxicillin
Double?cross?linked sodium alginate/chitosan hydrogels were synthesized using calcium chloride and glutaraldehyde as the cross?linking agents. The hydrogels are pH sensitive with swelling characteristics composition dependent. Amoxicillin incorporated in an optimal hydrogel composition was shown to be released by the diffusion?swelling?controlled time?dependent non?Fickian process and showed excellent antibacterial activity against Gram?positive and Gram?negative bacteria.This research aims to fabricate a potent pH?sensitive double network sodium alginate/chitosan hydrogel cross?linked by calcium chloride (CaCl2) and glutaraldehyde for the controlled release of amoxicillin (AMX) to mitigate gastrointestinal tract bacterial infection. The effect of polymer ratios and CaCl2 concentration is investigated by the developing porosity, gel fraction, and swelling ratios in simulated physiological fluids of different pH and in vitro biodegradation at pH?7.4. Interaction between the polymers with the formation of cross?linked structures, amorphous phase nature, good thermal stability, and transition from porous, fibrous structures to highly densified structures of the hydrogels is revealed by scanning electron microscopy, Fourier?transform infrared spectroscopy, x?ray diffraction, and thermogravimetric analysis. Based on structure–property relationships, a sodium alginate/chitosan hydrogel (weight ratio 75:25) cross?linked with 2% CaCl2 and soaked in 2% (25 wt/v% solutions) glutaraldehyde is chosen for the incorporation of 200?mg of the drug. The percent cumulative AMX release in physiological fluids and the drug release kinetics using different models reveal that the most appropriate Korsmeyer–Peppas model suggests AMX release from the matrix follows diffusion coupled with swelling?regulated time?dependent non?Fickian transport process related to hydrogel erosion. Excellent antibacterial against Streptococcus pyogenes and Escherichia coli is exhibited by this composition.
» Publication Date: 03/07/2023
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement Nº 768737
