Integrated manufacturing of REciclable multi-material COmposites for the TRANSport sector
Transcriptome profiling of Microbacterium resistens MZT7 reveals mechanisms of 17?-estradiol response and biotransformation
17?-estradiol (E2) pollution has attracted much attention, and the existence of E2 poses certain risks to the environment and human health. However, the mechanism of microbial degradation of E2 remains unclear. In this study, the location of E2-degrading enzymes was investigated, and transcriptome analysis of Microbacterium resistens MZT7 (M. resistens MZT7) exposed to E2. The degradation of E2 by M. resistens MZT7 was via the biological action of E2-induced intracellular enzymes. With the RNA sequencing, we found 1109 differentially expressed genes (DEGs). Among them, 773 genes were up-regulated and 336 genes were down-regulated. The results of the RNA sequencing indicated the DEGs were related to transport, metabolism, and stress response. Genes for transport, transmembrane transport, oxidoreductase activity, ATPase activity, transporter activity and quorum sensing were up-regulated. Genes for the tricarboxylic acid cycle, ribosome, oxidative phosphorylation and carbon metabolism were down-regulated. In addition, heterologous expression of one enzymes efficiently degraded E2. These findings provide some new insights into the molecular mechanism of biotransformation of E2 by M. resistens MZT7.
» Author: Peng Hao, Zongshuo Lv, Sicheng Wu, Xiqing Zhang, Changlong Gou, Lixia Wang, Yanbin Zhu, Wangdui Basang, Yunhang Gao
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement Nº 768737
