Integrated manufacturing of REciclable multi-material COmposites for the TRANSport sector
Efficient biosynthesis of ??aminoadipic acid via lysine catabolism in Escherichia coli
??Aminoadipic acid (AAA) is a non?proteinogenic amino acid with potential application in pharmaceutical, chemical and animal feed industries. Currently, AAA is produced by chemical synthesis, which suffers from high cost and low production efficiency. In this study, we engineered Escherichia coli for high?level AAA production by coupling lysine biosynthesis and degradation pathways. First, the lysine?ketoglutarate reductase and saccharopine dehydrogenase from Saccharomyces cerevisiae and ??aminoadipate???semialdehyde dehydrogenase from Rhodococcus erythropolis were selected by in vitro enzyme assays for pathway assembly. Subsequently, lysine supply was enhanced by blocking its degradation pathway, overexpressing key pathway enzymes and improving NADPH regeneration. Finally, a glutamate transporter from Corynebacterium glutamicum was introduced to elevate AAA efflux. The final strain produced 2.94 and 5.64 g/L AAA in shake flasks and bioreactors, respectively. This work provides an efficient and sustainable way for AAA production.This article is protected by copyright. All rights reserved.
» Publication Date: 12/10/2022
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement Nº 768737
