Integrated manufacturing of REciclable multi-material COmposites for the TRANSport sector
ENGINEERED MICROORGANISMS FOR THE DECONSTRUCTION OF POLYMERS
Disclosed herein are engineered P. putida KT2440 co-expressing PETase and MHETase enzymes that selectively degrades PET into monomers, ethylene glycol and terephthalate (TPA). In another embodiment, disclosed herein are methods for making and using a highly efficient EG metabolizing P. putida KT2440 strain. Given that native P. putida does not have a TPA metabolic pathway, nor the proteins to transport TPA into the cell, the next metabolic engineering challenge for developing synthetic P. putida strain to plastic upcycling was enabling TPA catabolism in P. putida KT2440. TPA transporters and catabolic pathway have been characterized in several microorganisms including Comamonas sp. strain E6 and Rhodococcus jostii RHA1.
» Number: WO2019222396A1 (A1)
» Publication Date: 21/11/2019
» Applicant: ALLIANCE SUSTAINABLE ENERGY?[US]; UT BATTELLE LLC?[US]
» Inventor: BECKHAM GREGG TYLER?[US]; JAYAKODY LAHIRU N?[US] (4)
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement Nº 768737
